The Identification of an Unknown Plasmid using Restriction Enzymes Gel Electrophoresis and Transformation
The Identification of an Unknown Plasmid using Restriction Enzymes, Gel Electrophoresis, and Transformation.
Abstract
Using DNA technology, including restriction enzymes, gel electrophoresis, and transformation we performed two experiments to identify an unknown plasmid. The first experiment involved gel electrophoresis of plasmid DNA with and with out restriction enzymes to determine migration and number of base pairs in specific fragments. Known DNA fragments were used to determine the size of the unknown fragments. Plasmid maps of …
Is this Essay helpful? Join now to read this particular paper
and access over 480,000 just like this GET BETTER GRADES
and access over 480,000 just like this GET BETTER GRADES
…technique.
Bibliography
1.) Australian Biotechnology Association. What is Genetic Engineering? Australian Biotechnology Association Home Page. Hotbot. http://www.aba.asn.au/leaf2.html
2.) Campbell, Neil. 1996. Biology, 4th edition. p.338-339, 369 Benjamin/Cummings Publishing Co., Inc. New York.
3.) Johnson, Shea. Recombinant DNA. Student Reports of BI 220 University of Oregon. Infoseek. http://ponderosa-pine.uoregon.edu/Bi220/Johnson/menu.html
4.) Lawrence, S.M., M.K. Heidemann, and D.O. Straney. 2000. Biological Sciences 111L Laboratory Manual, 4th edition. p.141-148
Need a custom written paper? Let our professional writers save your time.