Describe the Process of Genetic Fingerprinting.
Date Submitted: 09/10/2006 02:07:42
Category: / History / European History
Length: 1 pages (215 words)
Category: / History / European History
Length: 1 pages (215 words)
Genetic Fingerprinting.
It has been found in research that sections of DNA which do not code for part of a gene contains highly repetitive sequences of bases. These are called Variable Number Tandem Repeats, {VNTR's}. A genetic fingerprint is a method of revealing the differences in size of VNTR's between individuals. Firstly a DNA sample is extracted from the cell. Restriction enzymes then cut sections of the DNA close to but not within the VNTR …
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…by capillary motion. The DNA fragments are then denatured by heating to give single stranded DNA. A radioactive isotope of phosphorus with a base sequence identical to one of the VNTR sequences is used to locate the particular bands. This is also known as a DNA probe and it binds to its complementary single stranded DNA. Any excess probe is washed off. The probes are accurately located by placing X-ray film over the nylon membrane.
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