Chromatin Isolation
Chromatin Isolation
DNA is the blueprint for all living things. DNA is made of a material called chromatin. The purpose of this experiment was to attempt to isolate, or separate, chromatin from onion cells. My lab partner was Dave Sugg.
Hypothesis: We should be able to isolate DNA from onion cells.
Procedure: To begin, weigh out about 15 grams of onion cubes and place it in a 400 ml beaker. Add about 60 ml of homogenizing medium to …
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…a key process in science. To extract DNA, three things need to be done. The DNA must be accessible (homogenization), the DNA must uncoil and become extractable, as well as separate itself from the proteins (deproteinization), and the DNA must become a visible, individual layer and therefore be able to be extracted (precipitation). This experiment could be improved if we had more time to do it. In general, though, everything in the experiment went smoothly.
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