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Science Coursework Trypsin Investigation Aim         I am going to prove that temperature effects the rate in which the enzyme Trypsin breaks down milk substrate into amino acids. Investigation "We strongly recommend the use of Vanish Stain Removal Tablets in conjunction with a 900 spin wash at 70°C"         This was found on a milk carton of a well-known drinks company, Nesquick. The major food group constituent of milk is, of course, milk. For my Science Investigation, I …

…a wider temperature range and tested other enzymes such as amylase or lipase to see if these gave the same results.         The only difficulty I had during my experiment, was keeping the solutions to constant temperatures. No changes would be made to my method if I was to do it again. Using different enzymes in my investigation would make my conclusion certain because I would get an accurate average optimum temperature for each enzyme tested.