The Determination of Optimal Temperature, pH, and the Reaction Rate for Alpha-Amylase.
ABSTRACT
The enzyme alpha-amylase is a catalyst to break up starch to from glucose. Without being consumed in the reaction, enzymes are catalysts that speed up the rate of the reaction. They achieve this by combining with a substrate and then separating from it form products. Environment factors that affect enzyme reactions include temperature and pH. The optimal temperature and pH at which the reaction will occur coincides with the point where the rate of …
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…the sample was removed heat escaped the cuvette. Also, the timing required rapid removals and transfers, the samples may have varied from 5ml, changing not only the indicator dilution, but also the reading of the spectrophotometer.
LITERATURE CITED
Campbell, N.A. 1996. Biology, 4th ed. Benjamin/Cummings Publishing Co.,
Redwood City, California.
Vliet, K.A. (ed.). 2000. A Laboratory Manual for Integrated Principles of Biology:
Part One – BSC 2010L. Pearson Custom Publishing, Needham Heights,
Massachusetts.
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**Bibliography**
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