Knock out mutants
Basic summary on knock-outs
In vitro, a DNA fragment is altered by insertion of a fragment containing a selectable marker. The interrupted gene is introduced into the cells. In some cells, homologous recombination results in the replacement of the resident “gene” with the interrupted gene.
Homozygous transgenic cells are often referred to as “knock-outs”. These knock-outs can then be examined to determine what, if any, phenotype is associated with mutations in the targeted gene.
If …
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…single restriction site of the rpoS cloned in the low copy number plasmid (B), which has ends compatible with EcoRI. The resulting plasmid (X) can then be confirmed to contain the rpoS::selectable marker construct by DNA sequencing.
Sources used:
Elias, F. Abdallah, James L. Bono,James A. Carroll, Philip Stewart, Kit Tilly, and Patricia Rosa. 2000. Altered Stationary-Phase Response in a Borrelia
burgdorferi rpoS Mutant. Journal of Bacteriology, May 2000, p. 2909-2918, Vol. 182, No. 10
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**Bibliography**
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