Knock out mutants
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…single restriction site of the rpoS cloned in the low copy number plasmid (B), which has ends compatible with EcoRI. The resulting plasmid (X) can then be confirmed to contain the rpoS::selectable marker construct by DNA sequencing.
Sources used:
Elias, F. Abdallah, James L. Bono,James A. Carroll, Philip Stewart, Kit Tilly, and Patricia Rosa. 2000. Altered Stationary-Phase Response in a Borrelia
burgdorferi rpoS Mutant. Journal of Bacteriology, May 2000, p. 2909-2918, Vol. 182, No. 10
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