Determination of an unknown amino acid from a titration curve
Date Submitted: 01/10/2003 09:16:51
Category: / Science & Technology / Chemistry
Length: 6 pages (1634 words)
Category: / Science & Technology / Chemistry
Length: 6 pages (1634 words)
Abstract
Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial
pH of the solution was 1.96, and the pKa's found experimentally were 2.0, 4.0, and 9.85. The accepted
pKa values were found to be 2.10, 4.07, and 9.47. The molecular weight was calculated to be 176.3 while
the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be
glutamic acid, hydrochloride.
Introduction
Amino acids are simple monomers which are …
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…equivalence points and the D volume (used in calculating the molecular weight). The half-equivalence
points were directly used to find the pKa values of the unknown. The molecular weight could also be
calculated. This data led to the determination of the identity of the unknown amino acid--glutamic acid,
hydrochloride.
References
Jicha, D.; Hasset, K. Experiments in General Chemistry; Hunt: Dubuque, 1991:37-53.
Kotz, J.C.: Treichel , P. Jr. Chemistry and Chemical Reactivity; Harcourt-Brace: Fort Worth, 1996; 816- 837.
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